EB3-EGFP and cherry-tubulin dynamics in a NIH3T3 cell

NIH3T3 cells expressing EGFP-a-tubulin (upper) or EGFP-EB1 (lower)

EB3-EGFP dynamics in a cultured hippocampla neurons 18DIV

Hippocampal neuron (18DIV) expressing EGFP-EB3 to track MT plus ends

Maximum intensity projection of a time-lapse showing EB3-EGFP comet motility relative to stable vGlut1-mCherry labeled boutons from an axon of a cultured hippocampal neuron pretreated with AP5 followed by a washout and incubation with bicuculline.

Maximum intensity projection of a time-lapse showing EB3-EGFP comet motility relativeo stable vGlut1-mCherry labeled boutons from axons of cultured hippocampal neurons infected with shNC control or shHAUS7 lentiviruses for 7d.

Representative time-lapse of EB3-EGFP comet motion relative to stable vGlut1-cherry labeled boutons from an axon of a rat hippocampal neuron in culture. Arrows show EB3 comets appearing from a vGlut+ bouton and moving distally until they fall off. 

Maximum intensity projection of a time-lapse showing EB3-EGFP comet motility invading into dendritic spines of cultured hippocampal neurons (28DIV) expressing dsRed cytosolic filler.

Maximum intensity projection of a time-lapse showing EB3-EGFP comet motility invading into a dendritic spine of a cultured hippocampal neuron (28DIV) expressing dsRed cytosolic filler.

Representative time-lapse of EB3-EGFP comet motion relative to stable vGlut1-cherry labeled boutons from an axon in the CA1 region of a mouse acute hippocampal slice. Arrows show EB3 comets appearing from a vGlut+ bouton and moving distally until they fall off. 

Mito-dsRed dynamics in axons of cultured mouse DRG neurons infected with shNC lentivirus.

Mito-dsRed dynamics in axons of cultured mouse DRG neurons infected with control shNC lentivirus plus 100 nM Bort (24 h) 

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